A comprehensive whole-brain atlas of cell types in the mouse

Allen Institute
RFA-MH-17-225
2017
1U19MH114830-01
Zeng, Hongkui

The large number of cells in the brain and the complexity of their molecular and functional characteristics make it difficult to define individual cell types. Zeng and colleagues plan to complement high throughput droplet-based transcriptome survey with deep sequencing technique and multiplexed error-robust fluorescence in situ hybridization (MERFISH) to comprehensively characterize gene expression information from anatomically mapped cells across the entire mouse brain. Additionally, they will use patch clamp method to measure neuronal function in specific brain regions, and combine electrophysiological with transcriptomic and morphological information to provide integrative profiles of individual cell types. These efforts will refine how we define cell types and will produce a census of individual cells in the mouse brain that can then be targeted for further study.

Allen Institute
United States
47° 37' 30.6552" N, 122° 20' 20.7312" W
US
9416011
Funded Status: 
Complete