A Molecular and Cellular Atlas of the Marmoset Brain

Massachusetts Institute Of Technology
RFA-MH-17-210
2017
1U01MH114819-01
Feng, Guoping
Although rodents are a highly accessible model and relatively simple to use for genetic studies, it is unclear whether the cell types found in rodent brains match those of primates. To help fill the evolutionary gap in knowledge between rodents and humans, Feng will lead a team to classify cells across the marmoset brain. They will use high-throughput single-cell RNA sequencing to identify cell types in the prefrontal cortex, striatum, and thalamus and will then spatially map the cell types they find in the brain using multiplexed error-robust in situ hybridization (MERFISH). By combining MERFISH with viral expression of marker proteins in subsets of neurons, the team will also correlate cell morphology with genetic information. Altogether these efforts will produce a census of cell types in the marmoset brain, which will be valuable information for future work into the genetics and circuits of the primate brain.
Massachusetts Institute Of Technology
United States
42° 21' 36.3276" N, 71° 5' 38.976" W
US
9415538
Funded Status: 
Active