Large-field-of-view high-throughput two-photon endoscope to image neuronal activity
Project Summary Large-field-of-view high-throughput two-photon endoscope to image neuronal activity Development of miniaturized optical endoscopes have enabled visualization and recording of neural activity in freely-behaving animals. Two-photon endoscopes have excellent signal-to-background ratio, and can image deep into the tissue. It has a good optical sectioning and low phototoxicity. However, two-photon endoscopes have a very limited field of view and imaging throughput, and cannot typically perform axial refocusing for 3D imaging.