Tools for Gene Editing in Marmosets
SUMMARY
The goal of this proposal is to create an efficient new set of tools and techniques for the generation of
genetically modified marmosets suitable for use as models of brain physiology and function.
Funded Awards
The National Institutes of Health (NIH) BRAIN Initiative funds a wide-variety of research: toolmakers, trainees, individual labs testing new hypotheses, and large, team-based efforts aiming to catalyze neuroscience inquiry forward. Explore NIH BRAIN Initiative funded awards listed below. Click on the project title to learn more about it within NIH RePORTER.
To see more NIH-funded awards and associated publications, please visit the NIH RePORTER.
Title
Investigator(s)
Institution
Fiscal Year
Funding Opportunity #
Project #
TitleTools for gene editing in marmosets
Investigator
Richard L Huganir, Christopher A Ross, James H. Segars, Xiaoqin Wang
Institute
johns hopkins university
Fiscal Year
Funding Opportunities Number
Project Number
TitleTracking the emergence of internal models
Investigator
Elizabeth A Buffalo, Adrienne L Fairhall
Institute
university of washington
Fiscal Year
Funding Opportunities Number
Project Number
Abstract
Central to human and animal cognition is the idea of internal models: an internal repository of
knowledge about the structure of the world and its affordances that enables prediction and
planning. The existence of such models is fundamental to experience.
TitleTwo-photon all-optical electrophysiology in behaving mice
Investigator
Adam Ezra Cohen
Institute
harvard university
Fiscal Year
Funding Opportunities Number
Project Number
PROJECT SUMMARY/ABSTRACT
Two-photon all-optical electrophysiology in behaving mice Neurons communicate through electrical signals, so the ability to record membrane potential from dozens or hundreds of points simultaneously within the brain of a behaving animal would be a transformative capability f
TitleUltra-fast cerebral blood flow imaging for quantifying brain dynamics
Investigator
Jia Guo
Institute
university of california riverside
Fiscal Year
Funding Opportunities Number
Project Number
Abstract/Project Summary
Blood oxygenation level dependent (BOLD) fMRI is widely used in neuroscience studies.
TitleUltrafast high-contrast voltage imaging in freely moving animals
Investigator
Jerome Mertz
Institute
boston university (charles river campus)
Fiscal Year
Funding Opportunities Number
Project Number
ABSTRACT
We propose to develop a targeted illumination confocal (TICO) microscope to enable high speed,
large-scale voltage imaging in the brain. This microscope will be based on the combination of two
key strategies. The first strategy is high-speed confocal microscopy based on line scanning.
TitleUnderstanding feedforward and feedback signaling between neuronal populations
Investigator
Adam Kohn, Christian Machens, Byron M. Yu
Institute
albert einstein college of medicine
Fiscal Year
Funding Opportunities Number
Project Number
Summary
Most perceptual, cognitive, and motor functions rely on neuronal activity distributed across multiple networks,
often located in different brain areas.
TitleUnderstanding how cortex supports flexible sensory representations
Investigator
Katherine Charlotte Wood
Institute
university of pennsylvania
Fiscal Year
Funding Opportunities Number
Project Number
Project Summary
Learning is a fundamental function of the brain: sensory representations must be flexible to adjust to changes in
environmental demands and experience, thus allowing us to adapt to the world around us.
TitleUnlocking the potential of High-speed widefield Imaging
Investigator
Luke Satish Kumar Theogarajan
Institute
university of california santa barbara
Fiscal Year
Funding Opportunities Number
Project Number
Project Summary:
Widefield imaging and spatial multiplexing are crucial to advancing the field of
neuroscience. Current imagers do not offer the speed and versatility needed for
calcium or voltage imaging experiments.
TitleVirtual observatory of the cortex: organelles, cells, circuits, and dynamics
Investigator
Forrest Christie Collman, Nuno Macarico Da Costa, R Clay Reid, Hyunjune Sebastian Seung
Institute
allen institute
Fiscal Year
Funding Opportunities Number
Project Number
We propose to create VOrtex, a Virtual Observatory for the Cortex: Spanning the Scales of Organelles, Cells,
Circuits, and Dynamics.
TitleWearable modular high-density diffuse optical tomography
Investigator
Jason Wayne Trobaugh
Institute
esperimage llc
Fiscal Year
Funding Opportunities Number
Project Number
Project Summary:
EsperImage, a startup company out of Washington University (WUSTL), will develop high fidelity, wearable,
optical technology that transcends limitations of both previous optical neuroimaging and magnetic resonance
imaging (MRI) tools to provide naturalistic brain imaging in adults a
TitleWearable RF-EEG Cap for closed loopTMS/fMRI/EEG Applications
Investigator
Lucia Isabel Navarro De Lara
Institute
massachusetts general hospital
Fiscal Year
Funding Opportunities Number
Project Number
Project Summary
Functional MRI (fMRI) is the prevailing method for both basic research and clinical functional neuroimaging in
humans.
TitleWhole-Brain Functional Imaging and Analysis of Zebrafish Sleep
Investigator
Geoffrey J Goodhill, David Aaron Prober, Thai V. Truong
Institute
california institute of technology
Fiscal Year
Funding Opportunities Number
Project Number
ABSTRACT
Sleep occupies a third of our lives and sleep-related ailments cost an estimated $100 billion per year, yet the
mechanisms governing its regulation remain poorly understood.
Title3D Functional Photoacoustic Imaging of Human Brain with a Stretchable Ultrasound Matrix Array
Investigator
Yun Jing
Institute
pennsylvania state university, the
Fiscal Year
Funding Opportunities Number
Project Number
Abstract
Many scientific efforts have been devoted to understanding the brain functions, and the relevance of its dynamics
during development, aging, and in diseased conditions. Alterations of the brain functions can result from
multifactorial processes and be reflected by various biomarkers.
TitleA 3D multimodal micron-scale human brain atlas bridging single cell data, neuropathology and neuroradiology
Investigator
Partha Pratim Mitra, Jiangyang Zhang
Institute
cold spring harbor laboratory
Fiscal Year
Funding Opportunities Number
Project Number
Digitized reference brains, also referred to as Common Coordinate Frameworks (CCFs), together
with superposed atlas annotations, are of central importance to neuroscience. They bear the
same relation to neuroscience as do reference genomes and genome annotations to cellular and
molecular biology.
TitleA cellular atlas of the primate and human basal ganglia
Investigator
Jason Daniel Buenrostro, Fei Chen, Gordon J Fishell, Evan Z Macosko
Institute
broad institute, inc.
Fiscal Year
Funding Opportunities Number
Project Number
PROJECT SUMMARY
The human basal ganglia (BG) are a collection of subcortical regions whose diverse, specialized cell types
influence motor control, emotional regulation, habit formation, and higher cognition.
TitleA community-driven development of the brain imaging data standard (BIDS) to describe macroscopic brain connections
Investigator
Franco Pestilli
Institute
university of texas at austin
Fiscal Year
Funding Opportunities Number
Project Number
Project Summary/Abstract
The Brain Imaging Data Structure (BIDS) is a BRAIN initiative (R24 MH114705)
community-driven standard meant to maximize neuroimaging data sharing, and facilitate analysis tool
development.
TitleA Computational Framework for Distributed Registration of Massive Neuroscience Images
Investigator
Matthew Mccormick
Institute
kitware, inc.
Fiscal Year
Funding Opportunities Number
Project Number
Project Summary
Neuroscience stands at the precipice of a new depth of understanding about how the brain works thanks to recent
advances in imaging data acquisition technologies such as light-sheet fluorescence microscopy (LSFM).
TitleA Genetic Engineering Toolbox for Marmosets (GETMarm): Development and optimization of genome editing and assisted reproduction techniques for marmoset models
Investigator
Guoping Feng
Institute
massachusetts institute of technology
Fiscal Year
Funding Opportunities Number
Project Number
PROJECT SUMMARY
While mice are essential models for many areas of neuroscience, there are also many aspects of
higher brain function and dysfunction that cannot be adequately modeled in rodents. Thus, there is a need for
new genetic models that have brain structure and function closer to humans.
TitleA harmonized vendor-agnostic environment for multi-site functional MRI studies
Investigator
Jon-Fredrik Nielsen, Maxim Zaitsev
Institute
university of michigan at ann arbor
Fiscal Year
Funding Opportunities Number
Project Number
Since its invention in the early 90s, functional magnetic resonance imaging (fMRI) has revolutionized our un-
derstanding of the human brain. Functional MRI may be used to observe brain function during a specific motor
or cognitive task, or at “rest” (resting-state fMRI).
TitleA MEMS-Based High-Throughput Photostimulation Device with Commercial Backplane Integration
Investigator
Janelle Claire Shane
Institute
boulder nonlinear systems, inc.
Fiscal Year
Funding Opportunities Number
Project Number
Boulder Nonlinear Systems (BNS) and Prof. Rikky Muller at UC-Berkeley propose a two-phase effort to address current speed limitations
in holographic photostimulation.